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Critical role of monocytes to support normal B cell and diffuse large B cell lymphoma survival and proliferation
Author(s) -
Mueller Chris G.,
Boix Charlotte,
Kwan WingHong,
Daussy Cécile,
Fournier Emilie,
Fridman Wolf H.,
Molina Thierry J.
Publication year - 2007
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0706481
Subject(s) - naive b cell , biology , b cell , cd40 , cell growth , ccl5 , chemokine , cd14 , cancer research , b cell lymphoma , monocyte , b cell activating factor , microbiology and biotechnology , immunology , lymphoma , t cell , antigen presenting cell , il 2 receptor , flow cytometry , antibody , inflammation , cytotoxic t cell , immune system , in vitro , biochemistry , genetics
Large B cell lymphomas can comprise numerous CD14 + cells in the tumor stroma, which raises the question of whether monocytes can support B cell survival and proliferation. We show that the coculture of monocytes with B cells from peripheral blood or from diffuse large B cell lymphoma enabled prolonged B cell survival. Under these conditions, diffuse large lymphoma B cells proliferated, and addition of B cell‐activating factor of the TNF family (BAFF) and IL‐2 enhanced cell division. Monocytes and dendritic cells (DC) had similar antiapoptotic activity on healthy B cells but displayed differences with respect to B cell proliferation. Monocytes and cord blood‐derived CD14 + cells promoted B cell proliferation in the presence of an anti‐CD40 stimulus, whereas DC supported B cell proliferation when activated through the BCR. DC and CD14 + cells were able to induce plasmocyte differentiation. When B cells were activated via the BCR or CD40, they released the leukocyte attractant CCL5, and this chemokine is one of the main chemokines expressed in diffuse large B cell lymphoma. The data support the notion that large B cell lymphoma recruit monocytes via CCL5 to support B cell survival and proliferation.