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Phagocytosis stimulates mobilization and shedding of intracellular CD16A in human monocytes and macrophages: inhibition by HIV‐1 infection
Author(s) -
Webster Nicole L.,
Kedzierska Katherine,
Azzam Rula,
Paukovics Geza,
Wilson John,
Crowe Suzanne M.,
Jaworowski Anthony
Publication year - 2006
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0705382
Subject(s) - phagocytosis , biology , monocyte , intracellular , macrophage , immunology , virus , granulocyte , microbiology and biotechnology , in vitro , virology , biochemistry
Surface and intracellular staining coupled with flow cytometric analysis was used to show for the first time that human macrophages and a minor subset of peripheral blood monocytes have an internal pool of CD16A, which is mobilized and shed during Fc receptor for immunoglobulin G‐mediated phagocytosis. Human immunodeficiency virus type 1 (HIV‐1) infection of monocyte‐derived macrophages in vitro led to a reduction in the phagocytosis‐induced up‐regulation in CD16A shedding. These results suggest that monocytes and macrophages may be a source of soluble CD16A, which is elevated in the serum of patients in a variety of disease states and that the mobilization and shedding of CD16A in response to phagocytosis are disrupted by HIV‐1 infection.