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Relevance of DC‐SIGN in DC‐induced T cell proliferation
Author(s) -
Gijzen Karlijn,
Tacken Paul J.,
Zimmerman Aukje,
Joosten Ben,
Vries I. Jolanda M.,
Figdor Carl G.,
Torensma Ruurd
Publication year - 2007
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0606414
Subject(s) - dc sign , sign (mathematics) , t cell , dendritic cell , biology , cell growth , monoclonal antibody , microbiology and biotechnology , immunology , biophysics , antibody , antigen , biochemistry , mathematics , immune system , mathematical analysis
The role of dendritic cell‐specific ICAM‐3‐grabbing nonintegrin (DC‐SIGN) in DC‐T cell communication was assessed by analyzing the effect of DC‐SIGN‐blocking mAb in MLR. The results show that the degree of inhibition by DC‐SIGN and LFA‐1 mAb depends on the magnitude of the MLR and the maturation status of the DC. Addition of DC‐SIGN mAb at several time‐points during MLR showed that DC‐SIGN is involved early on in DC‐T cell contacts. This initial role is masked by strong adhesive and costimulatory mechanisms, indicating a short‐lived effect of DC‐SIGN in DC‐T cell interactions. To examine this concept in more detail, the percentage of PBL capable of binding DC‐SIGN was determined. Analysis of several donors revealed that 1–20% PBL bind to beads coated with recombinant DC‐SIGN, and the DC‐SIGN‐binding cells comprised all major cell subsets found in blood. PBL isolated from a donor with high DC‐SIGN‐binding capacity were more prone to blocking by DC‐SIGN mAb in MLR than PBL from a donor with low DC‐SIGN‐binding capacity. This study indicates an initial and transient role for DC‐SIGN in T cell proliferation, which becomes apparent when T cell proliferation is low and when the percentage of DC‐SIGN binding PBL is high.