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Human immature monocyte‐derived dendritic cells produce and secrete α‐defensins 1–3
Author(s) -
RodríguezGarcía Marta,
Oliva Harold,
Climent Núria,
García Felipe,
Gatell José M.,
Gallart Teresa
Publication year - 2007
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0507295
Subject(s) - biology , proinflammatory cytokine , innate immune system , immunology , secretion , monocyte , acquired immune system , cytokine , immunity , microbiology and biotechnology , dendritic cell , immune system , inflammation , biochemistry
Defensins are effector molecules of the innate immunity with a broad antimicrobial spectrum, including HIV. They also link innate and adaptive immunity, displaying chemotactic activity for monocytes, T cells, and dendritic cells (DCs). α‐Defensins 1–3 are mainly produced by neutrophils, but their production by other leukocyte subsets has also been reported. Herein, we studied whether monocyte‐derived DCs (MDDCs), which are regarded as a model for myeloid DCs, produce α‐defensins 1–3. We found that immature MDDCs (imMDDCs) produce α‐defensins 1–3 mRNA, but this production is undetectable or barely detectable following 48 h of maturation with the proinflammatory cytokine cocktail (IL‐1β+IL‐6+TNF‐α) or LPS. It is surprising that α‐defensins 1–3 production was up‐regulated when exposed to each one of the proinflammatory cytokines alone, especially IL‐1β. α‐Defensins 1–3 produced by imMDDCs were mainly secreted peptides. Production and secretion of α‐defensins 1–3 by imMDDCs can have biological relevance for the antigen processing of pathogens and can contribute to understanding differences in susceptibility to infections, an issue of special interest in the field of HIV infection.