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CD5‐low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells
Author(s) -
Huang YiChun,
Hung ShaoWen,
Jan TongRong,
Liao KuangWen,
Cheng ChiungHsiang,
Wang YuShan,
Chu ReaMin
Publication year - 2008
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0408255
Subject(s) - perforin , biology , interleukin 21 , cd16 , cytotoxic t cell , nkg2d , lymphokine activated killer cell , microbiology and biotechnology , granzyme b , cd8 , cd5 , interleukin 12 , granzyme , natural killer cell , granzyme a , population , cd49b , cd3 , immunology , antigen , flow cytometry , in vitro , medicine , biochemistry , environmental health
NK cell markers and receptors have been discovered in many mammalian species, such as humans, mice, rats, pigs, and cows. However, there is still a lack of information concerning NK cell markers or receptors in canines. We have discovered that canine CD5‐low density (CD5 lo ) cells in PBL are closely associated with NK cell characteristics. CD5 lo cells comprised 14.9 ± 6.68% of the total PBL. A high proportion of the CD5 lo cell population expressed CD3 (96.6%), CD8α (77.7%), CD8β (53%), α/β TCR (83%), and CD11/18 (80%), but the expression of γ/δ TCR (6.5%), CD4 (10.6%), and CD21 (2.4%) was low. CD5 lo cells were larger than CD5‐high density (CD5 hi ) cells. Light and electron microscopy revealed numerous large cytoplasmic granules in CD5 lo cells, especially after IL‐2 stimulation, which was in contrast to CD5 hi , in which intracytoplasmic granules were not frequently seen. After IL‐2 stimulation, CD5 lo cells had significantly stronger NK cytotoxicity than CD5 hi cells. CD5 lo cells had much higher mRNA levels for NKG2D, CD16, CD94, CD160, perforin, and granzyme than CD5 hi . Following IL‐2 stimulation, CD5 lo cells had significantly higher mRNA levels of NKp30, NKp44, CD16, and CD94 than CD5 hi cells. In addition, IL‐2‐stimulated, CD5 lo ‐depleted PBL showed a loss of NK cytotoxicity. CD5 lo cells also showed significantly lower antigen‐specific cytotoxic T cell activity as compared with CD5 hi cells. Taken together, the CD5 lo subset in canine PBL is closely related to canine NK cells, and CD5 lo can be used as a phenotypic marker for an IL‐2‐dependent canine NK cell enrichment.