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MHC II molecules and invariant chain reside in membranes distinct from conventional lipid rafts
Author(s) -
Karacsonyi Claudia,
Bedke Tanja,
Hinrichsen Nils,
Schwinzer Reinhard,
Lindner Robert
Publication year - 2005
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0405189
Subject(s) - major histocompatibility complex , biology , lipid raft , mhc class ii , membrane , mhc class i , cd74 , t cell , microbiology and biotechnology , antigen , biochemistry , immune system , immunology
Major histocompatibility complex class II (MHC II) peptide complexes can associate with lipid rafts, and this is a prerequisite for their recruitment to the immunological synapse and for efficient T cell stimulation. One of the most often used criterion for raft association is the resistance to extraction by the detergent Triton X‐100 (TX‐100) at low temperature. For MHC II, a variety of detergents have been used under different conditions, leading to variable and often conflicting conclusions about the association of MHC II with detergent‐resistant membranes (DRMs). To clarify whether these inconsistencies were caused by variations in the isolation protocols or reflect different biochemical properties of MHC II lipid complexes, we used two standardized procedures for the isolation of membranes resistant to TX‐100, 3‐[(3‐cholamidopropyl)dimethylammonio]‐1‐propanesulfonate (CHAPS), or Brij 98. Our results suggest that some of the reported variations in the association of MHC II with DRMs are caused by differences in the methods. We also show that in our hands, specific and efficient flotation of MHC II and the MHC II‐associated invariant chain from mouse B‐lymphoma cells was only achieved with Brij 98, but not with TX‐100 and CHAPS. We furthermore used DRMs prepared from hen egg lysozyme‐fed B‐lymphoma cells to activate the T cell hybridoma 3A9. In agreement with our biochemical data, T cell activation could only be achieved with Brij 98‐ but not with TX‐100‐resistant membranes. Thus, MHC II and also the invariant chain belong to a set of proteins comprising the T cell receptor, prominin, and the prion protein, which reside in membrane environments distinct from conventional lipid rafts.