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Recent thymic origin, differentiation, and turnover of regulatory T cells
Author(s) -
Mabarrack Nicholas H. E.,
Turner Nicole L.,
Mayrhofer Graham
Publication year - 2008
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0308201
Subject(s) - foxp3 , biology , il 2 receptor , immunology , thymectomy , microbiology and biotechnology , homeostasis , regulatory t cell , endocrinology , medicine , t cell , immune system , myasthenia gravis
Regulatory CD4 + T cells (T reg ) are essential to maintain self‐tolerance. Release of natural T reg from the thymus is believed to commence soon after birth, but it is unclear how many are produced by “conversion” in the periphery, whether numbers are maintained after puberty by general homeostatic mechanisms that regulate lymphocyte numbers, or whether significant numbers are produced by the involuted thymus. To address the origin of T reg in normal adult rats, we focused on recent thymus emigrants (RTE). Approximately 30% of CD4 + CD25 + forkhead box p3 (Foxp3) + T reg expressed markers associated with RTE. Following thymectomy, numbers of cells expressing these markers fell by 80% within 30 days. Furthermore, although only ∼5% of CD4 + single‐positive thymocytes expressed Foxp3 within 24 h after intrathymic injection of FITC, more than 30% of the labeled CD4 + RTE were Foxp3 + , suggesting that some RTE may acquire Foxp3 in the periphery. Thus, some RTE may acquire Foxp3 rapidly after emigration from the thymus. T reg are dividing rapidly with apparent half‐lives of ∼18 days and ∼7 days for the CD4 + CD25 + Foxp3 + and CD4 + CD25 − Foxp3 + subsets, respectively. The apparently slower turnover of CD4 + CD25 + Foxp3 + cells is a result of CD4 + CD25 + Foxp3 + → CD4 + CD25 − Foxp3 + conversion, with no loss of regulatory function. Taken together, the data suggest that T reg in adults are relatively short‐lived and that their numbers are maintained by rapid cell division and continuous replenishment from the thymus.