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Postnatal maturation of total cell content and up‐regulated surface expression of Mac‐1 (CD11b/CD18) in polymorphonuclear leukocytes of human infants
Author(s) -
Storm Shawn W.,
Mariscalco M. Michele,
Tosi Michael F.
Publication year - 2008
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0308170
Subject(s) - cd11a , cd18 , biology , integrin alpha m , flow cytometry , immunology , andrology , immunofluorescence , granulocyte , cell , in vitro , endocrinology , medicine , antibody , biochemistry
Markedly deficient expression of membrane‐activated complex 1 (Mac‐1; CD11b/CD18) by polymorphonuclear neutrophils (PMN) of human neonates compared with adults is well documented. To define postnatal maturation of Mac‐1 expression of PMN, lysates of PMN from 21 infants, aged 1–14 months, and concurrent adult controls were assayed by ELISA for total cell content of Mac‐1 and LFA‐1 (CD11a/CD18), and LFA‐1 content was within the normal adult range at all ages tested. Mac‐1 content was ∼50% of adult levels for infants 1–2 months of age and steadily increased to reach normal adult levels by 11–12 months of age. For a separate group of 25 infants, aged 0.5–11 months, measurement of surface expression of Mac‐1 and LFA‐1 on activated PMN by immunofluorescence flow cytometry yielded results that were similar to those obtained by ELISA.