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T cells traffic from brain to cervical lymph nodes via the cribroid plate and the nasal mucosa
Author(s) -
Goldmann Jana,
Kwidzinski Erik,
Brandt Christine,
Mahlo Jacqueline,
Richter Daniel,
Bechmann Ingo
Publication year - 2006
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0306176
Subject(s) - biology , cervical lymph nodes , mucous membrane of nose , lymph , olfactory mucosa , anatomy , pathology , neuroscience , genetics , medicine , cancer , metastasis , olfactory system
Although drainage pathways of soluble antigens from brain to cervical lymph nodes have been well established, there is no direct evidence for similar routes of leukocytes leaving the central nervous system. We developed a protocol allowing the cross‐sectioning of an entire head‐neck preparation while preserving the signal of the GFP. We monitored how GFP‐expressing CD4 T lymphocytes injected into the entorhinal cortex after lesion or the lateral ventricle of unlesioned C57/bl6 mice reach cervical lymph nodes. Irrespective of the injection site, we demonstrate their passage through the cribroid plate, appearance in the nasal mucosa, and specific accumulation in one of the cervical lymph nodes.