z-logo
Premium
The extracellular matrix protein mindin regulates trafficking of murine eosinophils into the airspace
Author(s) -
Li Zhuowei,
Garantziotis Stavros,
Jia Wei,
Potts Erin N.,
Lalani Sikander,
Liu Zhi,
He YouWen,
Foster W. Michael,
Hollingsworth John W.
Publication year - 2009
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0208135
Subject(s) - immunology , eosinophil , biology , eotaxin , asthma
Asthma remains a major cause of morbidity and hospitalizations in developed nations. Despite the widespread prevalence of this disease, the genetic and environmental factors that mediate development and progression of allergic airways disease remain poorly understood. Pulmonary recruitment of eosinophils is believed to contribute to many cardinal features of allergic airways disease. Therefore, it is paramount to understand host factors that contribute to pulmonary eosinophil recruitment into the lungs. Mindin is a component of pulmonary extracellular matrix, which can regulate inflammatory cell recruitment. We characterized the role of mindin in the severity of allergic airways disease using established murine models. There were no baseline differences in wild‐type and mindin‐deficient animals in cell counts or airway physiology. Using the OVA murine model of allergic airways disease, we observed that mindin‐deficient animals have less‐severe allergic airways disease with fewer airspace eosinophils and lower lung‐lavage levels of inflammatory Th 2 cytokines such as IL‐13 and IL‐4. Furthermore, mindin‐deficient animals have reduced airway hyper‐responsiveness after methacholine challenge. To determine the role of mindin in eosinophil trafficking, independent of antigen immunization or T lymphocyte activation, we instilled IL‐13 directly into the lungs of mice. In this model, mindin regulates eosinophil recruitment into the airspace. In vitro experiments demonstrate that mindin can enhance eotaxin‐mediated eosinophil adhesion and migration, which are dependent on the expression of integrins α M β 2 and α 4 β 1 . In conclusion, these data suggest that mindin participates in integrin‐dependent trafficking of eosinophils and can contribute to the severity of allergic airways disease.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here