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cDNA microarray analysis reveals fundamental differences in the expression profiles of primary human monocytes, monocyte‐derived macrophages, and alveolar macrophages
Author(s) -
Li Jiangning,
Pritchard David K.,
Wang Xi,
Park David R.,
Bumgarner Roger E.,
Schwartz Stephen M.,
Liles W. Conrad
Publication year - 2007
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0206124
Subject(s) - biology , gene expression , complementary dna , gene , microarray analysis techniques , gene expression profiling , monocyte , bronchoalveolar lavage , microarray , dna microarray , microbiology and biotechnology , immunology , genetics , lung , medicine
We report the systematic use of large‐scale cDNA microarrays to study the gene expression profiles of primary human peripheral blood monocytes (MONO) in comparison with in vitro‐differentiated, M‐CSF‐induced MONO‐derived macrophages (MAC) and primary human alveolar MAC (AM), obtained by bronchoalveolar lavage from the lungs of normal volunteers. These studies revealed large‐scale differences in the gene expression profile between both MAC types (MAC and AM) and MONO. In addition, large differences were observed in the gene expression profiles of the two MAC types. Specifically, 21% of genes on the array (2904 out of 13,582) were differentially expressed between AM and MONO, and 2229 out of 13,583 probes were differentially expressed between MAC and AM. Our expression data show remarkable differences in gene expression between different MAC subpopulations and emphasize the heterogeneity of different MAC populations. This study underscores the need to scrutinize models of MAC biology for relevance to specific disease processes.