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Differential expression and function of IgA receptors (CD89 and CD71) during maturation of dendritic cells
Author(s) -
Pasquier Benoit,
Lepelletier Yves,
Baude Cédric,
Hermine Olivier,
Monteiro Renato C.
Publication year - 2004
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0204101
Subject(s) - biology , transferrin receptor , microbiology and biotechnology , immune system , receptor , cd86 , dendritic cell , antigen , scavenger receptor , cd40 , immunology , major histocompatibility complex , mhc class ii , t cell , cytotoxic t cell , in vitro , endocrinology , lipoprotein , biochemistry , cholesterol
Dendritic cells (DC) are the most efficient antigen‐presenting cells residing in mainly peripheral tissues. Antigen uptake by DC is particularly efficient, being mediated by various receptors such as lectin, scavenger receptors, and Fc receptors (FcRs). Immunoglobulin A (IgA) is part of the first‐line immune barrier in mucosae, where DC are numerous. A member of the FcR family, FcαRI, is expressed on interstitial DC. We report here that monocyte‐derived DC (Mo‐DC) express another IgA receptor (IgA‐R), the transferrin receptor (TfR), even in the absence of DC proliferation in vitro. Upon incubation with inflammatory cytokines such as tumor necrosis factor α and interleukin (IL)‐1β or maturating agents (lipopolysaccharide, CD40 ligand), FcαRI and TfR expression on Mo‐DC was specifically up‐regulated, whereas FcγRs and FcɛRI expression was down‐regulated. Both IgA‐Rs were functional, being able to mediate endocytosis by immature and activated Mo‐DC. Although FcαRI internalized IgA complexes on both types of DC, TfR was only able to mediate IgA complex internalization by immature cells. Cross‐linking of FcαRI but not of TfR resulted in up‐regulation of major histocompatibility complex (MHC) class II/CD86 expression and secretion of IL‐10 and IL‐12 by immature Mo‐DC. Moreover, in activated Mo‐DC, cross‐linking of FcαRI could up‐regulated MHC class II/CD86 and triggered IL‐10 secretion. Our findings led us to propose that FcαRI expressed by interstitial‐type DC could play a critical role to sample IgA‐recognized antigens and also during DC activation.