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Pivotal Advance: Eosinophil infiltration of solid tumors is an early and persistent inflammatory host response
Author(s) -
Cormier Stephania A.,
Taranova Anna G.,
Bedient Carrie,
Nguyen Thanh,
Protheroe Cheryl,
Pero Ralph,
Dimina Dawn,
Ochkur Sergei I.,
O’Neill Katie,
Colbert Dana,
Lombari Theresa R.,
Constant Stephanie,
McGarry Michael P.,
Lee James J.,
Lee Nancy A.
Publication year - 2006
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0106027
Subject(s) - eosinophil , biology , eosinophilia , infiltration (hvac) , immune system , adoptive cell transfer , immunology , major basic protein , melanoma , tumor necrosis factor alpha , degranulation , eosinophil peroxidase , pathology , cancer research , t cell , medicine , biochemistry , physics , receptor , asthma , thermodynamics
Tumor‐associated eosinophilia has been observed in numerous human cancers and several tumor models in animals; however, the details surrounding this eosinophilia remain largely undefined and anecdotal. We used a B16‐F10 melanoma cell injection model to demonstrate that eosinophil infiltration of tumors occurred from the earliest palpable stages with significant accumulations only in the necrotic and capsule regions. Furthermore, the presence of diffuse extracellular matrix staining for eosinophil major basic protein was restricted to the necrotic areas of tumors, indicating that eosinophil degranulation was limited to this region. Antibody‐mediated depletion of CD4 + T cells and adoptive transfer of eosinophils suggested, respectively, that the accumulation of eosinophils is not associated with T helper cell type 2‐dependent immune responses and that recruitment is a dynamic, ongoing process, occurring throughout tumor growth. Ex vivo migration studies have identified what appears to be a novel chemotactic factor(s) released by stressed/dying melanoma cells, suggesting that the accumulation of eosinophils in tumors occurs, in part, through a unique mechanism dependent on a signal(s) released from areas of necrosis. Collectively, these studies demonstrate that the infiltration of tumors by eosinophils is an early and persistent response that is spatial‐restricted. It is more important that these data also show that the mechanism(s) that elicit this host response occur, independent of immune surveillance, suggesting that eosinophils are part of an early inflammatory reaction at the site of tumorigenesis.