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Human monocyte‐derived dendritic cells express TLR9 and react directly to the CpG‐A oligonucleotide D19
Author(s) -
Hoene Victoria,
Peiser Matthias,
Wanner Reinhard
Publication year - 2006
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0106011
Subject(s) - biology , tlr9 , cpg oligodeoxynucleotide , cd86 , microbiology and biotechnology , dendritic cell , cd40 , cpg site , immunology , t cell , immune system , gene , cytotoxic t cell , genetics , dna methylation , gene expression , in vitro
Oligodeoxynucleotides (ODNs) containing unmethylated CpG exhibit their immunostimulatory activities by binding to TLR. Here, we show that human monocyte‐derived dendritic cells (moDC) contain TLR9 protein, surprisingly, in amounts comparable with plasmacytoid DC (pDC). Immature moDC but not mature moDC nor monocytes captured CpG‐ODNs. moDC stimulation with the CpG‐A ODN D19 up‐regulated CD83, CD86, and HLA‐DR. Without CD40 ligand costimulation, full maturation was not achieved. D19‐stimulated moDC primed allogeneic CD4 + ‐T cells for proliferation and differentiation into IFN‐γ‐secreting Th1 cells. Neither IL‐12 nor IL‐6 or TNF‐α was involved. Microarray analysis pointed to a participation of Type I IFNs. In fact, D19‐stimulated moDC secreted considerable amounts of IFN‐α. This indicates that moDC themselves sense viral and bacterial DNA and do not need help from pDC.