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TNF revisited: TNF‐independent antitumor activity in sera of mice sensitized with Propionibacterium acnes and challenged with lipopolysaccharide
Author(s) -
Schwamberger Günter,
Hammerl Peter,
Ferber Ernst,
Freudenberg Marina,
Galanos Chris
Publication year - 2003
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1189/jlb.0103042
Subject(s) - tumor necrosis factor alpha , in vivo , lipopolysaccharide , propionibacterium acnes , biology , in vitro , effector , cytotoxic t cell , immunology , cytokine , cytotoxicity , microbiology and biotechnology , cancer research , bacteria , biochemistry , genetics
Sera of mice sensitized with bacteria and subsequently challenged with lipopolysaccharide promote hemorrhagic necrosis of tumors in vivo and display cytotoxic activity against tumor cells in vitro, which has been attributed to the induction of tumor necrosis factor (TNF). Here, we describe the induction of a previously unrecognized antitumor activity in such sera, which is distinct from TNF but displays tumor‐specific cytocidal activity in vitro as well as potent tumor‐regressing activity in vivo. Biochemical analysis of this activity yielded a molecular mass of ∼150 kDa, closely resembling a novel tumoricidal factor of murine macrophages (Mφ) termed MTC 170 (Mφ tumor cytotoxin, approximate molecular mass 170 kDa), which we have previously proposed to constitute a major effector pathway for the destruction of tumor cells by activated Mφ.