Open AccessAbstracts from the 11th Symposium on Experimental Rhinology and Immunology of the Nose (SERIN 2017)
Author(s) -
Gracia Ibon Eguiluz,
Rondón Carmen,
Campo Paloma,
Prieto Ana,
Mayorga Lina,
Galindo Luisa,
Molina Ana,
Blanca Miguel,
Torres Maria Jose,
Morikawa Taiyo,
Fukuoka Ayumi,
Matsushita Kazufumi,
Fujieda Shigeharu,
Yoshimoto Tomohiro,
Iwasaki Naruhito,
Smulders Tamar,
Van Egmond Danielle,
Van Drunen Kees,
Van Der Schee Marc,
Beule Achim Georg,
Berings Margot,
Jult Anton,
Vermeulen Hanne,
De Ruyck Natalie,
Derycke Lara,
Ucar Hakan,
Ghekiere Philip,
Temmerman Robin,
Ellis John,
Bachert Claus,
Lambrecht Bart,
Dullaers Melissa,
Gevaert Philippe,
Arasi Stefania,
Perna Serena,
Resch Yvonne,
Lupinek Christian,
Chen KuanWei,
Vrtala Susanne,
Valenta Rudolf,
Matricardi Paolo Maria,
Gonçalves Ivânia,
Jacinto Tiago,
Amaral Rita,
Pereira Ana M.,
Araújo Luís M.,
Couto Mariana,
Fonseca João A.,
Stjarne Par,
Kaulsay Ranbir,
Pohl Wolfgang,
Plaza Maria Carmen,
Prieto Ana Maria,
Mayorga Cristobalina,
Herknerova Magdalena,
Wang Tengchin,
Wu Chiejun,
Kilimajer Jonathan,
Pujols Laura,
RocaFerrer Jordi,
Callejas Borja,
FuentesPrado Mireya,
PerezGonzalez Maria,
Alobid Isam,
Valero Antonio,
Picado Cesar,
Murray Ruth,
Mullol Joaquim,
Steelant Brecht,
Martens Katleen,
Boeckxstaens Guy,
Seys Sven F.,
Hellings Peter W.,
Biggs Timothy C.,
Hayes Stephen M.,
Harries Philip G.,
Pender Sylvia,
Salib Rami J.,
Kim Jean,
Lee Hyun Sil,
Kalogjera Livije,
Vrkic Nada,
Topic Anita,
Tomljenovic Dejan,
Greguric Tomislav,
Radovanovic Patricija Bankovic,
Jund Rainer,
Haimerl Pascal,
Chaker Adam M.,
Schober Yvonne,
Schindela Sonja,
Nockher Andreas,
SchmidtWeber Carsten B.,
Bieren Julia EsserVon,
Ickrath Pascal,
Kleinsasser Norbert,
Beyersdorf Niklas,
Ding Xin,
Hagen Rudolf,
Hackenberg Stephan,
Cangiano Daniela,
Cinetto Francesco,
Brescia Giuseppe,
Marioni Gino,
Zanotti Claudia,
Schiavon Franco,
Padoan Roberto,
Caputo Ilaria,
Neri Raffaella,
Agostini Carlo,
Kim Ji Heui,
Jang Yong Ju,
Lim Ji Youn,
Kim Sung Hee,
Savlevich Elena,
Gaganov Leonid,
Kochnova Maria,
Egorov Victor,
Fok Jie Shen,
Hanif Tanzeela,
Renkonen Jutta,
Joenväärä Sakari,
Kankainen Matti,
Mäkelä Mika,
Kauppi Paula,
Pelkonen Anna,
Mattila Pirkko,
Renkonen Risto,
ToppilaSalmi Sanna,
Holtappels Gabriele,
Lambrecht Bart N.,
BlancaLópez Natalia,
GonzalezVisiedo Miguel,
Jurado Raquel,
Canto Gabriela
Publication year - 2017
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/s13601-017-0163-x
Subject(s) - medicine , rhinology , allergy , asthma , population , vernal keratoconjunctivitis , spirometry , allergic conjunctivitis , cohort , dermatology , immunology , surgery , otorhinolaryngology , environmental health
Eosinophilic granulomatosis with polyangiitis (EGPA)\udis an uncommon systemic necrotizing vasculitis that affects small to\udmedium sized vessels and is associated with severe asthma, allergic\udrhinitis, nasal polyposis and blood and tissue eosinophilic infiltration.\udAntineutrophil cytoplasmic autoantibodies (ANCA) are present\udin about 40% of cases. The presence of four or more of the above\uddescribed findings yields a sensitivity of 85% and a specificity of 99.7% for the final diagnosis of EGPA. The aim of the study was to develop a\udnew diagnostic tool to support the diagnostic and prognostic work-up\udof EGPA patients with the possibility to evaluate extracellular mediators\udin nasal secretion.\udMethods: Nasal secretions were gained from 40 patients of which 20\udEGPA, 10 suspected EGPA and 10 controls after positioning the cotton\udpieces in the nasal middle meatus where they were left for 10 min.\udSubsequently the cotton pieces were put in 5 ml of saline solution and\udleft in ice until processing. The liquid obtained from the squeezing of\udthe cottons was centrifuged two times at 1600 RPM for 10 min. Supernatant\udwas frozen after first centrifugation. Trypan Blue was added\udto 10 μl of sample to calculate the number of cells and their vitality.\udFinally, 100 μl of sample with containing 1.5–2.00 × 105 cells were centrifuged\udwith Cytospin at 300 RPM for 15 min. The slides were stained\udwith May Grunwald/Giemsa and cell population analysed at microscope\udwith objective oil immersion 40× of magnification. The remaining\udcells were frozen or cultured.\udResults: The mean number of cells obtained after centrifugation was\ud2.00–4.08 × 106 cells/ml for EGPA patients and 6.42–8.62 × 105 cells/\udml for control group and suspected EGPA. Cytologic analysis allowed\udus to count an increase in the percentage of eosinophils in EGPA\udpatients with active ENT disease and in some of the patients with a\udsuspect of EGPA, where this percentage correlated with histologic evidence\udof disease.\udConclusions: This method seems preliminarily a reliable cytologic\udexamination allowing fresh cells isolation and analysis of extracellular\udmediators. Compared to the direct slither of the mucus on the surface\udof the glass, cytospin significantly reduced any dye accumulation. Further\udstudies are ongoing to support the described method, in order to\udconfirm the cytologic diagnostic reliability to identify prognostic biomarkers\udof EGPA