Open AccessRETRACTED ARTICLE: Down-regulation of microRNA-26a and up-regulation of microRNA-27a contributes to aggressive progression of human osteosarcoma
Author(s) -
Afshin Taheriazam,
Reza Bahador,
Seyyed Hasan Karbasy,
Seyed Mir Mansoor Moazen Jamshidi,
Ali Torkaman,
Emad Yahaghi,
Mohammadreza Shakeri
Publication year - 2015
Publication title -
diagnostic pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.805
H-Index - 52
ISSN - 1746-1596
DOI - 10.1186/s13000-015-0400-3
Subject(s) - osteosarcoma , microrna , proportional hazards model , malignancy , pathology , medicine , survival analysis , cancer research , metastasis , oncology , biology , cancer , gene , biochemistry
Background Osteosarcoma is the most common primary bone malignancy with high local aggressiveness and rapid metastasizing potential, resulting in poor survival . Increasing reports suggest that deregulated microRNAs (miRNAs) might provide novel therapeutic targets for cancers. However, the expression of miR-26a and miR-27a in osteosarcoma need further investigation in clinical samples. In our study, we evaluate the expression of these miRNAs in osteosarcoma tissues and compared with paired adjacent non-tumor bone tissues using RT-qPCR. Methods Total RNA was purified from patients with osteosarcoma and noncancerous bone tissues. Real-time PCR was applied to quantify the expression level of miR-26a and miR-27a. Moreover, the correlation of these markers with clinicopathological characteristics was also evaluated in osteosarcoma patients. A cox proportional hazards model was performed to assess multivariate analyses of prognostic values. Results Our result suggested that miR-26aexpression level in osteosarcoma bone tissue was significantly lower than that in the paired noncancerous bone tissues. MiR-27a expression was higher in osteosarcoma bone tissue in comparison with paired noncancerous bone tissues. The results indicated that low expression level of miR-26a and high expression of miR-27a were associated with high TNM stage ( P = 0.001; P = 0.012), tumor grade ( P = 0.007; P = 0.016), and distant metastasis ( P = 0.004; P = 0.001). Kaplan-Meier analysis and log-rank test indicated that patients with low expression of miR-26a and high expression of miR-27a had shorter overall survival (log-rank test: P < 0.001). Multivariate Cox proportional hazards model analysis showed that low expression of miR-26a and high expression of miR-27a ( P = 0.021; P = 0.011), high TNM stage ( P = 0.001; P = 0.003), tumor grade ( P = 0.005; P = 0.01), and distant metastasis.( P = 0.002; P = 0.005) were independent prognostic factors for overall survival patients with osteosarcoma cancer. Conclusions In conclusion, our findings suggested that expression level of miR-26a and miR-27a contributes to aggressive progression of this malignancy. Therefore, may have clinical potentials as a non-invasive diagnostic/prognostic biomarker for osteosarcoma patients.