Open AccessExpression profiling of nasal polyp epithelial cells identifies two distinct phenotypes and suggests a role for neurogenic inflammation
Author(s) -
Cornet Marjolein,
Kristina Kostamo,
Bas Rinia,
Koos Zwinderman,
Danielle Egmond,
Esther Groot,
Wytske Fokkens,
Cornelis Drunen
Publication year - 2015
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-5-s4-o5
Subject(s) - nasal polyps , inflammation , phenotype , medicine , pathology , mucous membrane of nose , epithelium , immunology , immunohistochemistry , pathophysiology , gene expression profiling , biology , gene expression , gene , genetics
Method Primary epithelial cells were isolated from nasal polyps of 24 affected individuals and from the middle turbinates of 9 healthy controls. After a limited culture period RNA was extracted and the expression profile determined using Human Genome U133 Plus 2.0 Genechip Array (Affymetrix inc., Santa Clara, CA, USA). Supernatants collected from the epithelial cells and immunohistochemistry on biopsies were used for validation.