Open AccessThe non‐specific lipid transfer protein from hazelnut, Cor a 8, a relevant food allergen
Author(s) -
Dubiela Pawel,
Pfeifer Sabine,
Bublin Merima,
Hafner Cristine,
HoffmannSommergruber Karin
Publication year - 2015
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-5-s3-p17
Subject(s) - plant lipid transfer proteins , epitope , allergen , edman degradation , digestion (alchemy) , chemistry , immunoglobulin e , food allergy , biochemistry , chromatography , allergy , antibody , peptide sequence , biology , immunology , gene
Methods nCor a 8 was purified from raw hazelnuts by precipitation and chromatographical steps. Subsequently, the protein was identified by N-terminal sequencing and mass spectrometry. Stability of the protein was investigated by simulated gastric and duodenal digestion assays. Hazelnut lipids were extracted employing the hexane method. Finally, IgE binding activity of native and reduced/alkylated nCor a 8 was tested in ELISA using sera from 6 hazelnut allergic patients.