Open AccessCytokine gene polymorphisms in Greek patients with severe asthma
Author(s) -
Papakosta Despina,
Kontakioti Eirini,
Domvri Kalliopi,
Fouka Evagelia,
Ioannidou Despina,
Porpodis Konstantinos,
Daniilidis Michail,
Zarogoulidis Konstantinos
Publication year - 2015
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-5-s2-p9
Subject(s) - asthma , medicine , group b , group a , genotype , interleukin 4 , immunology , gastroenterology , interleukin 13 , allergy , polymorphism (computer science) , cytokine , gene , genetics , biology
Method The study population consisted of 52 patients, 30 with allergic asthma, mean age 35,2±14,51 years (Group A), 22 patients with non-allergic asthma, mean age 47,1 ±16,3 years (Group B) and 21 healthy controls, mean age 36,6±10,5 years (Group C). 29 patients had mild, 10 moderate and 12 severe asthma. For the immunogenetic study DNA was extracted from the patients’ peripheral blood samples. Determination of gene polymorphisms were performed by PCR using the INVITROGEN kit.