Open AccessSimilar activation of sputum granulocytes in eosinophilic and non‐eosinophilic asthma
Author(s) -
Hilvering Bart,
Tak Tamar,
Tesselaar Kiki,
Koenderman Leo
Publication year - 2015
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-5-s2-o8
Subject(s) - sputum , medicine , eosinophilic , eosinophil , immunology , degranulation , asthma , eosinophil cationic protein , eosinophilia , pathology , receptor , tuberculosis
Method We obtained peripheral blood and induced sputum from 21 asthma patients and defined two patient groups based on sputum eosinophilia (cut-off 3%). Eosinophils and neutrophils from blood and sputum were analysed by flow cytometry for expression of activation and degranulation markers. Data were analysed by both classical, non-parametric statistics and a more advanced multi-dimensional approach, using principal component analysis (PCA).