Open AccessThe HLA library for drug screening in preventing severe drug hypersensitivity
Author(s) -
Wei Chunyu
Publication year - 2014
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-4-s3-p51
Subject(s) - abacavir , medicine , carbamazepine , oxcarbazepine , human leukocyte antigen , pharmacology , drug , lamotrigine , allopurinol , pharmacogenomics , immunology , antigen , epilepsy , lamivudine , hepatitis b virus , virus , psychiatry
Background The clinical and economic impact of severe drug hypersensitivity is huge for both healthcare and pharmaceutical industry; however, no method for predicting culprit candidate t drugs under clinical development exists currently. As genetic variants in human-leukocyte antigens (HLA) have been linked to inter-individual differences in the risk of drug hypersensitivity, we ascertained whether HLA library can serve as a drug screening tool for their potential to cause severe drug hypersensitivity. Method We cloned and generated stable cell lines expressing one common HLA allele, and then purified each HLA protein genetically linked to particular hypersensitivity, immobilized on a protein chip and analyzed the interaction between HLA and drugs by surface plasmon resonance (SPR) analysis.