Open AccessEvaluation of immediate allergic reactions to dipyrone using dipyrone metabolites in basophil activation test
Author(s) -
Montanez Maria Isabel,
GarciaMartin Elena,
Ariza Adriana,
Torres Maria Jose,
Agundez Jose AG,
Mayorga Cristobalina,
Doña Inmaculada,
BlancaLopez Natalia,
Zambonino Maria A,
Andreu Inmaculada,
Blanca Miguel
Publication year - 2014
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-4-s3-p33
Subject(s) - basophil activation , provocation test , medicine , pyrazolones , drug , allergy , pharmacology , immunoglobulin e , drug allergy , hypersensitivity reaction , immunology , basophil , antibody , chemistry , alternative medicine , pathology , medicinal chemistry
Background Immediate selective hypersensitivity reactions to pyrazolones are thought to be mediated by specific-IgE antibodies. The diagnosis of selective reactions to pyrazolones is mainly based on the clinical history, skin testing and/ or drug provocation test (DPT). Skin testing has low sensitivity and has potential risk of eliciting an anaphylactic response as well as DPT. Therefore the use of in vitro tests could be a safe and straightforward approach, although till now no well-validated test is available. Basophil activation test (BAT) has been proposed as a new in vitro diagnostic tool for different drug reactions although its sensibility is not optimal. The aim of this study was to analyze the potential usefulness of dipyrone metabolites to improve BAT sensitivity.