Open AccessT cell activation in biological diagnosis of amoxicillin delayed hypersensitivity
Author(s) -
Chaisemartin Luc,
Lim Suzy,
Neukirch Catherine,
Thetis Marguerite,
NicaiseRoland Pascale,
Peltier Sandy,
CholletMartin Sylvie
Publication year - 2014
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-4-s3-p28
Subject(s) - elispot , amoxicillin , medicine , gold standard (test) , peripheral blood mononuclear cell , immunology , flow cytometry , context (archaeology) , in vitro , t cell , antibiotics , pharmacology , immune system , microbiology and biotechnology , biology , paleontology , biochemistry
Background Delayed hypersensitivity to betalactam drugs is one of the most frequently suspected drug allergies. However, it is sometimes difficult to diagnose. Drug challenges are the diagnostic gold standard, but they are costly and complex to organize. In vitro testing could thus represent a valid alternative. The existence of amoxicillin-specific T cells have long been demonstrated, however few studies have compared the diagnostic performances of the different methods aiming at detecting them, and there is currently no available test validated for diagnostic purpose. In this context, we started a prospective study to establish the relevance of such in vitro tests in delayed hypersensitivity to amoxicillin.