Open AccessAnalysis of Pru av 1.0101 protein expression in Prunus avium cultivars with ELISA and liquid chromatography/mass spectrometry
Author(s) -
Verschuren Martie,
Knaven Edward,
BastiaanNet Shanna,
Foetisch Kay,
Scheurer Stephan,
Noij Theo,
Wichers Harry
Publication year - 2014
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-4-s2-p5
Subject(s) - liquid chromatography–mass spectrometry , mass spectrometry , medicine , chromatography , prunus , protein expression , cultivar , biochemistry , botany , biology , chemistry , gene
Background Food allergy to sweet cherry in Europe is frequently associated with birch pollinosis. After ingestion of fresh cherries, oral allergy symptoms occur due to the cross reactivity of IgE to the birch pollen Bet v 1 homologous cherry protein Pru av 1. In order to detect natural occurring cherry cultivar variations, Pru av 1 protein expression needs to be analysed. However no commercial assays are available so far. Therefore, we determined the protein expression of isoallergen Pru av 1.0101 in five cherry cultivars using ELISA and liquid chromatography-tandem mass spectrometry (LC-MS/MS).