Open AccessLinear versus conformational epitopes of three cow's milk allergens
Author(s) -
Bogh Katrine Lindholm,
Madsen Jeanette Lund,
Kroghsbo Stine,
Madsen Charlotte Bernhard,
Barkholt Vibeke
Publication year - 2014
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-4-s2-p23
Subject(s) - milk allergy , adjuvant , medicine , antibody , allergy , immunoglobulin e , epitope , skimmed milk , food allergens , food allergy , immunology , milk protein , food science , biology
Method Specific antibodies were raised in a Brown Norway (BN) rat model of food allergy. BN rats were dosed either (1) intraperitoneally (i.p.) with the purified native cow’s milk allergens with or without the use of Al(HO)3 as adjuvant or (2) orally with skimmed milk powder without the use of adjuvant. The specific IgG1 and IgE antibody responses were analysed against native and denatured allergens by means of different ELISAs.