Open AccessThe role of n plant glycosylation in Act d 2 allergenicity
Author(s) -
DiazPerales Araceli,
GarridoArandia Maria,
MuruaGarcia Amaya,
Tordesillas Leticia,
GarciaCasado Cristina,
BlancaLópez Natalia,
Ramos Tania,
Canto Gabriela,
Blanco Carlos,
CuestaHerranz Javier,
Pacios Luis F,
SanchezMonge Rosa
Publication year - 2014
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-4-s2-p16
Subject(s) - proinflammatory cytokine , glycosylation , moiety , sensitization , sugar , glycan , medicine , incubation , immunology , immunoglobulin e , glycoprotein , biochemistry , chemistry , stereochemistry , inflammation , antibody
Results N-glycan and protein fractions were recognized by specific IgE of kiwi-allergic patients. By contrast, the sugar moiety showed a reduced capacity to activate basophils and T cells, but not dentritic cells derived of patient’s monocytes. In this sense, the proinflammatory cytokine production, measured as IL6 and IL10, was increased by the incubation of dendritic cells with the sugar moiety. Conclusions The sugar moiety plays a significant role in sensitization, inducing the activation of antigen presenting cells. Nevertheless, the protein fraction is the responsible for the allergic reactions.