Open AccessGlutathione‐s‐transferase is a minor allergen in birch pollen because of restricted release from hydrated pollen grains
Author(s) -
Deifl Stephan,
Zwicker Christian,
Vejvar Eva,
Kitzmüller Claudia,
Gadermaier Gabriele,
Nagl Birgit,
Vrtala Susanne,
Zlabinger Gerhard,
Briza Peter,
Ferreira Fatima,
Bohle Barbara
Publication year - 2014
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-4-s2-o1
Subject(s) - pollen , immunoglobulin e , allergen , basophil , allergy , basophil activation , immunology , house dust mite , antibody , cross reactivity , microbiology and biotechnology , medicine , biology , botany , cross reactions
Methods bGST was expressed in Escherichia coli, purified and characterized by mass spectrometry. BALB/c mice were immunized with bGST or Bet v 1. Antibody and T cell responses were assessed. 217 sera from birch pollen-allergic patients were tested for IgE-reactivity to bGST by ELISA. The allergenicity of bGST was evaluated with IgE-loaded rat basophil leukaemia cells (RBL) expressing the a-chain of the human receptor FceRI. Cross-reactivity of IgE between bGST and GST from house dust mite, Der p 8, was assessed with murine and human sera in ELISA. The release kinetics of bGST and Bet v 1 from birch pollen upon hydration were studied by immunoblotting.