Open AccessPre‐ and postnatal administration of Lactobacillus reuteri decreases TLR2 responses in infants
Author(s) -
Forsberg Anna,
Abrahamsson Thomas R,
Björkstén Bengt,
Jenmalm Maria C
Publication year - 2014
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-4-21
Subject(s) - tlr2 , probiotic , lactobacillus reuteri , lipoteichoic acid , medicine , immune system , immunology , innate immune system , lactobacillus rhamnosus , allergy , chemokine , peripheral blood mononuclear cell , receptor , toll like receptor , cytokine , microbiology and biotechnology , biology , bacteria , in vitro , staphylococcus aureus , biochemistry , genetics
Background Mice models indicate that intact Toll like receptor (TLR) signaling may be essential for the allergy protective effects of diverse bacterial exposure observed in clinical trials and epidemiological studies. Probiotic supplementation with Lactobacillus reuteri from pregnancy week 36 and to the infant through the first year of life decreased the prevalence of IgE‐associated eczema at two years ( ClinicalTrials.gov NCT01285830). The effect of this supplementation on innate immune responses to bacterial products and the expression of associated TLRs were explored. Methods Blood mononuclear cells were collected at birth, 6, 12 and 24 months from 61 infants and cultured with TLR2, 4 and 9 ligands. Cytokine and chemokine secretion was determined as well as TLR2, 4 and 9 mRNA expression. Results Probiotic supplementation was associated with decreased LTA (lipoteichoic acid) induced CCL4, CXCL8, IL‐1β and IL‐6 responses at 12 months and decreased CCL4 and IL‐1β secretion at 24 months. TLR2 mRNA expression was not affected by probiotic treatment. Conclusions Decreased responses to TLR2, the main receptor for LTA from Gram positive bacteria, in probiotic treated children seem to be dependent on factors downstream of TLR mRNA expression.