Open AccessIdentification and characterization of Lyc e 4, a Bet v 1 homologous PR‐10 protein, in tomato fruits
Author(s) -
Jamin A,
Wangorsch A,
Mahler V,
Malczyk AH,
Bartel D,
Foetisch K,
Lidholm J,
Vieths S,
Scheurer S
Publication year - 2013
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-3-s3-p87
Subject(s) - medicine , homologous chromosome , identification (biology) , microbiology and biotechnology , genetics , gene , botany , biology
Background Allergy to tomato (Lycopersicon esculentum) has been reported to occur in 3-16% of food allergic subjects. Tomato allergy is frequently manifested as OAS, urticaria and gastrointestinal symptoms. So far, three tomato allergens, Lyc e 1 (profilin), Lyc e 2 (invertase) and Lyc e 3 (nsLTP) are listed in the official IUIS allergen database. Although tomato allergy is frequently associated with birch pollen allergy in Central Europe, a cross-reactive Bet v 1 homologous allergen has not yet been described in tomato fruits. The objective of the present study was the cDNA cloning, recombinant expression and immunological characterization of Bet v 1 homologous PR10-proteins from tomato fruits.