Open AccessSequential IgE‐epitopes of ovomucoid exposed by glutathione are relevant in persistent egg allergy
Author(s) -
Kinaciyan T,
RothWalter F,
Starkl P,
Zuberbier T,
Brunner R,
PaliSchöll IPS,
Kinkel J,
Felix FF,
JensenJarolim E
Publication year - 2013
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-3-s3-p173
Subject(s) - medicine , allergy , egg allergy , immunoglobulin e , immunology , epitope , food allergy , antibody
Methods Reduced ovomucoid in raw eggwhite was detected using a fluorescence-labeled alkylation probe. The common antioxidants glutathione and cystein were used for in vitro linearization and effects were monitored by CD-spectrometry. Egg-allergic patients were tested serologically (n=19) and by skin prick test (n=9) for IgE against native and linearized ovomucoid and optionally its cooked state.