Open AccessMolecule‐based diagnosis of Apium graveolens allergy: is there a need to increase the current allergen panel?
Author(s) -
Gadermaier G,
Vejvar E,
Briza P,
HoffmannSommergruber K,
Hemmer W,
Ferreira F
Publication year - 2013
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-3-s3-o12
Subject(s) - medicine , apium graveolens , allergy , allergen , current (fluid) , dermatology , immunology , botany , biology , electrical engineering , engineering
Methods Immunoblots were performed with extracts of celeriac and celery stalks using sera of A. graveolens sensitized patients from Austria (n=23). Purified allergens were obtained from natural source (celeriac) or produced as recombinant proteins in E. coli and IgE reactivity to rApi g 1, rApi g 2, nApi g 5, and nApi g 6 was tested in ELISA. IgE cross-inhibition assays were performed with purified lipid transfer proteins (LTP). Celeriac extract was separated in 2D gel electrophoresis and IgE reactive spots were subjected to mass spectrometry-based analysis.