Open AccessThe role of mast cells, interleukin‐13 and transient receptor potential channels in a mouse model of chemical‐induced airway hyperresponsiveness
Author(s) -
Devos Fien,
De Vooght Vanessa,
Nemery Benoit,
Hoet Peter,
Vanoirbeek Jeroen
Publication year - 2013
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-3-s2-p31
Subject(s) - medicine , immunology , methacholine , transient receptor potential channel , trpv1 , mast cell , adoptive cell transfer , lung , inflammation , lymph , allergy , receptor , pharmacology , t cell , pathology , respiratory disease , immune system
Method On days 1 and 8, wild type C57Bl/6 mice, IL-13, TRP (Transient Receptor Potential) A1, TRPV1 and mast cell deficient mice were dermally sensitized with 1% TDI (toluene-2.4-diisocyanate) or vehicle (acetone/olive oil) on both ears. On day 15, the mice received a single intranasal challenge with 0.1% TDI or vehicle. In a second experiment TDI or vehicle sensitized wild type C57Bl/6 mice received an intraperitoneal injection of the NK1R antagonist RP67580 (1μg/μl) prior to the challenge. Airway reactivity to methacholine, lung inflammation, lymphocyte subpopulations in the draining auricular lymph nodes and total serum IgE were assessed 24h after the challenge.