Open AccessComparative proteomic analysis of tear fluid versus nasal mucus in allergic rhinitis patients and healthy controls
Author(s) -
Tomazic Peter Valentin,
BirnerGrünberger Ruth,
Obrist Britta,
Wolf Hanno,
LangLoidolt Doris
Publication year - 2013
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-3-s2-p18
Subject(s) - medicine , mucus , allergy , proteome , immunology , tears , mucin , albumin , allergic conjunctivitis , gastroenterology , pathology , bioinformatics , biology , ecology
Background Allergy is a common disorder in the western world with a prevalence of 15% to 20%. The combination of ocular symptoms in patients with allergic rhinitis occurs frequently. Nasal mucus for the nasal epithelium and tear fluid for the eye are the first defence barrier against various pathogens including aeroallergens. Little is known about the nasal mucus and particularly about the tear fluid proteome. The aim of the study was to analyse both body fluids on a proteome level and detect possible impact of its proteins in the pathophysiology in allergic rhinoconjunctivitis. Methods Fifty-eight patients (29 allergic, 29 healthy controls) were included in this study. Allergy status was confirmed through symptom history and skin prick test (SPT). Patients sensitized to house dust mite or animals were excluded. Nasal mucus was collected with a special suction device, tear fluid was collected with a glass capillary. Specimens then were sent for LC MS/MS mass spectrometry. Results