Open AccessAn assessment of cross reactivity with patients allergic to birch pollen using the immunoblotting
Author(s) -
ZbikowskaGotz Magdalena,
Ku¿miñski Andrzej,
JóŸwiak Jolanta,
Napiórkowska Katarzyna,
Fares Iwona,
Socha Ewa,
Rêdowicz Jolanta,
Bartuzi Zbigniew
Publication year - 2011
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-1-s1-p93
Subject(s) - pollen , allergen , allergy , cross reactivity , medicine , botany , immunology , cross reactions , biology , antigen
The aim Evaluation of the applicability of immunoblotting for diagnosing cross reactivity between homologous proteins present in various sources. Method and material: Group of 21 patients with birch pollen allergy aged 41,7±5,2 revealing symptoms after eating cross-reacting food and positive skin tests, or prick by prick tests with native food allergens. Control group 7 persons aged 39,8±4,7, allergic to birch pollen without any symptoms after having eaten the above mentioned food. Concentration of asIgE for birch pollen, apple, carrot and celery allergens has been measured with EIA method. Concentration of asIgE against recombinant allergens of birch pollen Bet v1, Bet v2 and celery Api g1 has been evaluated with FEIA-UniCAP 100. As plant material served specially prepared extracts of apples’, separately peel and pulp, as well as of carrot and celery. The detection of proteins following the immunobloting was conducted by incubating in the darkness the nitrocellulose membrane in a buffer solution and peroxidase substrate.