Open AccessAnaphylactic reactions to pomegranate: identification and characterization of eliciting IgE‐reactive components
Author(s) -
Petersen Arnd,
Kleinheinz Andreas,
Jappe Uta
Publication year - 2011
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-1-s1-p88
Subject(s) - medicine , immunoglobulin e , provocation test , erythema , ingestion , immunology , allergy , pollen , oral allergy syndrome , ambrosia , allergen , traditional medicine , dermatology , botany , biology , antibody , pathology , alternative medicine
Patients and methods A 26 year old female patient developed erythema, swelling of the ears and pruritus within 10 min after ingestion of pomegranate. She is allergic to birch pollen and apple. Prick and IgE tests revealed a positive reaction to mites, tree and grass pollen, ambrosia, mugwort and apple. Scratch test was positive for pomegranate, the seeds showing a stronger reaction than the juice. Oral provocation test with kiwi, peach and cherry was negative. The serum was analysed for IgE reactivity by Western blotting. As extracts served different sections of pomegranate (juice, extract and seed). IgE-reactive protein bands were analyzed and identified by protein sequencing and homology screening.