Open AccessBiochemical and immunological characterisation of Act d 10 a lipid transfer proteins from green kiwifruit
Author(s) -
Bernardi Maria Livia,
Giangrieco Ivana,
Camardella Laura,
Panico Maria Rosaria,
Zennaro Danila,
Ferrara Rosetta,
Palazzo Paola,
Tuppo Lisa,
Tamburrini Maurizio,
Carratore Vito,
Santoro Mario,
Ciardiello Maria Antonietta,
Mari Adriano
Publication year - 2011
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-1-s1-o23
Subject(s) - plant lipid transfer proteins , medicine , computational biology , immunology , biochemistry , biology , gene
Methods Pulp and seeds of green kiwifruit were manually separated and homogenized. Act d 10 was purified from seed extracts by ion exchange chromatography and RPHPLC, and sequenced by an automatic amino acid sequencer. Act d 10 was evaluated in vivo by skin prick test (SPT) and in vitro by detecting IgE using streptavidin-conjugated CAP (Phadia, Sweden). Act d 10 was spotted on a customized ISAC microarray (PMD, Austria) for evaluating IgE prevalence in a larger cohort of allergic patients.