Open AccessEffect of modification of peanut conglutin on IgE and T cell reactivity in adults with peanut allergy
Author(s) -
Hoffen Els,
Kleij Hanneke,
Jager Stans den Hartog,
Knol Edward,
Opstelten DirkJan,
Koppelman Stef,
Knulst André
Publication year - 2011
Publication title -
clinical and translational allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.979
H-Index - 37
ISSN - 2045-7022
DOI - 10.1186/2045-7022-1-s1-o16
Subject(s) - peanut allergy , medicine , allergy , immunoglobulin e , immunology , oral immunotherapy , food allergy , antibody
Methods Purified native peanut conglutin, containing Ara h2 and Ara h6, was chemically modified by reduction and alkylation (RA), without or with additional glutaraldehyde treatment (RAGA). Serum of 14 peanut allergic patients (DBPCFC-confirmed) was analyzed for peanut-specific antibodies by IgE immunoblot and IgE/IgG1/IgG4 ELISA. Allergen-induced basophil activation was analyzed by direct basophil activation test (BAT), using whole blood and washed whole blood to remove the plasma. Native conglutin-specific T cell lines were analyzed for proliferation and cytokine production.