Open AccessAutophagy of the ER Requires Actin Assembly Driven by the Interaction of ER with Endocytic Pits
Author(s) -
Peter Novick,
Dongmei Li,
Susan FerroNovick
Publication year - 2022
Publication title -
contact
Language(s) - English
Resource type - Journals
ISSN - 2515-2564
DOI - 10.1177/25152564221093215
Subject(s) - endocytic cycle , microbiology and biotechnology , autophagy , actin , endocytosis , cell cortex , chemistry , biology , cytoskeleton , biochemistry , receptor , cell , apoptosis
Autophagy of the cortical ER in budding yeast was unexpectedly found to require End3, a component of the endocytic machinery that promotes the assembly of actin at endocytic pits on the plasma membrane. The cortical ER transiently interacts with invaginating endocytic pits through a linkage consisting of VAP proteins, oxysterol binding proteins and type I myosins. These proteins are required for actin assembly and for autophagy of the ER. Assembly of actin at these contact sites may direct the movement of ER away from the cortex towards sites of autophagosome assembly.