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Vaccine Quality Ensured by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection
Author(s) -
Jeffrey S. Rohrer
Publication year - 2020
Publication title -
slas technology
Language(s) - English
Resource type - Journals
eISSN - 2472-6311
pISSN - 2472-6303
DOI - 10.1177/2472630319890309
Subject(s) - amperometry , polysaccharide , chemistry , conjugate , derivatization , chromatography , ion chromatography , microbiology and biotechnology , high performance liquid chromatography , biochemistry , biology , mathematical analysis , mathematics , electrode , electrochemistry
Many important vaccines use bacterial capsular polysaccharides, or shorter polysaccharides or oligosaccharides, derived from the capsular polysaccharides, conjugated to protein. It is imperative that manufacturers understand the carbohydrate composition of these vaccines and deliver a product with a consistent polysaccharide or polysaccharide conjugate composition and content. High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is a major technique used to understand the carbohydrate composition of these vaccines and ensure product quality. HPAE-PAD separates and detects carbohydrates without analyte derivatization. This paper describes the basics of the HPAE-PAD technique and then reviews how it has been applied to Haemophilus influenzae type b, pneumococcal, meningococcal, group B streptococcal, and Salmonella polysaccharide and corresponding conjugate vaccines.

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