Open AccessDrying Methods Alter Angiotensin-I Converting Enzyme Inhibitory Activity, Antioxidant Properties, and Phenolic Constituents of African Mistletoe (Loranthus bengwensis L) Leaves
Author(s) -
Ganiyu Oboh,
Olasunkanmi S. Omojokun,
Adedayo O. Ademiluyi
Publication year - 2016
Publication title -
journal of evidence-based complementary and alternative medicine
Language(s) - English
Resource type - Journals
eISSN - 2156-5872
pISSN - 2156-5899
DOI - 10.1177/2156587215600560
Subject(s) - antioxidant , chemistry , viscum album , lipid peroxidation , chromatography , enzyme , traditional medicine , food science , biochemistry , medicine
This study investigated the most appropriate drying method (sun drying, oven drying, or air drying) for mistletoe leaves obtained from almond tree. The phenolic constituents were characterized using high-performance liquid chromatography–diode array detector, while the inhibitory effect of the aqueous extracts of the leaves on angiotensin-I converting enzyme (ACE) was determined in vitro as also the antioxidant properties. Oven-dried extract (kidney [276.09 μg/mL] and lungs [303.41 μg/mL]) had the highest inhibitory effect on ACE, while air-dried mistletoe extract (kidney [304.47 μg/mL] and lungs [438.72 μg/mL]) had the least. Furthermore, the extracts dose-dependently inhibited Fe 2+ and sodium nitroprusside-induced lipid peroxidation in rat’s heart and kidney. Also, all extracts exhibited antioxidative properties as typified by their radical scavenging and Fe-chelating ability. Findings from this study revealed that oven drying is the best of the 3 drying methods used for mistletoe obtained from almond host tree, thus confirming that diversity in drying methods leads to variation in phenolic constituents and biological activity of plants.