Open AccessDetecting Streptococcus suis by nanopore sequencing in endophthalmitis: A case report
Author(s) -
YiYan Wang,
Qiong Huang,
Yang Cheng
Publication year - 2021
Publication title -
european journal of inflammation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.219
H-Index - 20
eISSN - 2058-7392
pISSN - 1721-727X
DOI - 10.1177/20587392211002657
Subject(s) - streptococcus suis , endophthalmitis , clostridium perfringens , nanopore sequencing , pathogen , microbiology and biotechnology , infectious disease (medical specialty) , biology , medicine , disease , dna sequencing , pathology , bacteria , gene , genetics , virulence
Endophthalmitis is a rare and infectious disease caused by Streptococcus suis (S suis). Traditionally, S suis is detected by the pathogenic microorganism culture method, which has low positivity and high false negativity. Nanopore sequencing (NS), which is a third-generation sequencing technology, has several advantages over the traditional method; in particular, it is cost and time effective and has a high throughput. In this report, a case of infectious endophthalmitis caused by trauma is examined. The NS results suggest that the pathogen in question is a mixed infection caused by S suis and Clostridium perfringens. This case report provides evidence of the fact that NS can quickly identify pathogens, which is of great significance for clinical diagnosis and treatment.