In vitro chondrogenic differentiation of human adipose-derived stem cells with silk scaffolds | Zendy

Hyeon Joo Kim | Zendy; SangHyug Park | Zendy; Jennah L. Durham | Zendy; Jeffrey M. Gimble | Zendy; David L. Kaplan | Zendy; Jason L. Dragoo | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

In vitro chondrogenic differentiation of human adipose-derived stem cells with silk scaffolds

Author(s) -

Hyeon Joo Kim,

SangHyug Park,

Jennah L. Durham,

Jeffrey M. Gimble,

David L. Kaplan,

Jason L. Dragoo

Publication year - 2012

Publication title -

journal of tissue engineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.436

H-Index - 30

ISSN - 2041-7314

DOI - 10.1177/2041731412466405

Subject(s) - chondrogenesis , adipose tissue , microbiology and biotechnology , stem cell , tissue engineering , chemistry , cellular differentiation , silk , chondrocyte , glycosaminoglycan , mesenchymal stem cell , scaffold , biomedical engineering , in vitro , materials science , biology , biochemistry , medicine , gene , composite material

Human adipose-derived stem cells have shown chondrogenicdifferentiation potential in cartilage tissue engineering in combination with naturaland synthetic biomaterials. In the present study, we hypothesized that porousaqueous-derived silk protein scaffolds would be suitable for chondrogenicdifferentiation of human adipose-derived stem cells. Human adipose-derived stem cellswere cultured up to 6 weeks, and cell proliferation and chondrogenic differentiationwere investigated and compared with those in conventional micromass culture. Cellproliferation, glycosaminoglycan, and collagen levels in aqueous-derived silk scaffoldswere significantly higher than in micromass culture. Transcript levels of SOX9 and typeII collagen were also upregulated in the cell–silk constructs at 6 weeks. Histologicalexamination revealed that the pores of the silk scaffolds were filled with cellsuniformly distributed. In addition, chondrocyte-specific lacunae formation was evidentand distributed in the both groups. The results suggest the biodegradable andbiocompatible three-dimensional aqueous-derived silk scaffolds provided an improvedenvironment for chondrogenic differentiation compared to micromass culture

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research