Open AccessProteomic Analysis of the Luteal Endometrial Secretome
Author(s) -
Jessica G. Scotchie,
Marc A. Fritz,
Mihaela M. Mocanu,
Bruce A. Lessey,
Steven L. Young
Publication year - 2009
Publication title -
reproductive sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 77
eISSN - 1933-7205
pISSN - 1933-7191
DOI - 10.1177/1933719109337165
Subject(s) - endometrium , secretory protein , difference gel electrophoresis , embryo , proteomics , luteinizing hormone , trypsin , gel electrophoresis , biology , two dimensional gel electrophoresis , andrology , luteal phase , microbiology and biotechnology , chemistry , medicine , secretion , endocrinology , biochemistry , hormone , gene , enzyme
Endometrium attains a secretory architecture in preparation for embryo implantation, but the identity of most endometrial secretory products remains unknown. Our objective was to characterize the endometrial secretome and compare protein expression between prereceptive (luteinizing hormone [LH]+4) and receptive (LH+9) phase endometrium. Endometrial lavage was performed in 11 participants and analyzed by difference gel electrophoresis (DIGE). LH+4 and LH+9 specimens were labeled with cyanine fluorescent dyes Cy3 and Cy5 tags, respectively, and combined. Proteins were separated using 2-dimensional gel electrophoresis, isolated, trypsin-digested, and subjected to mass spectrometry. In all, 152 proteins were identified; 82 were differentially expressed. Most proteins with increased expression on LH+9 functioned in host defense, while proteins with decreased expression had many functions. A total of 14 proteins had changes suggesting altered posttranslational modification. This article describes the first application of proteomic analysis to endometrial secretions, allowing identification of novel endometrial proteins as well as those differentially secreted in prereceptive and receptive phases.