Open AccessClinicopathological significance and underlying molecular mechanism of downregulation of basonuclin 1 expression in ovarian carcinoma
Author(s) -
Zi-Qian Liang,
Lu-Yang Zhong,
Jie Li,
Jin-Hai Shen,
Xin-Yue Tu,
Zheng-Hong Zhong,
Jingjing Zeng,
Junhong Chen,
ZhuXin Wei,
YiWu Dang,
Su-Hua Huang,
Gang Chen
Publication year - 2021
Publication title -
experimental biology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 146
eISSN - 1535-3702
pISSN - 1535-3699
DOI - 10.1177/15353702211052036
Subject(s) - downregulation and upregulation , ovarian carcinoma , mechanism (biology) , expression (computer science) , carcinoma , cancer research , clinical significance , pathology , biology , ovarian cancer , medicine , oncology , cancer , genetics , gene , computer science , physics , quantum mechanics , programming language
In this study, we aim to identify the clinical significance of basonuclin 1 ( BNC1 ) expression in ovarian carcinoma (OV) and to explore its latent mechanisms. Via integrating in-house tissue microarrays, gene chips, and RNA-sequencing data, we explored the expression and clinical value of BNC1 in OV. Immunohistochemical staining was utilized to confirm the protein expression status of BNC1. A combined SMD of -2.339 (95% CI : -3.649 to -1.028, P < 0.001) identified that BNC1 was downregulated based on 1346 samples, and the sROC (AUC = 0.93) showed a favorable discriminatory ability of BNC1 in OV patients. We used univariate and multivariate Cox regulation to evaluate the prognostic role of BNC1 for OV patients, and a combined hazard ratio of 0.717 (95% CI : 0.445-0.989, P < 0.001) revealed that BNC1 was a protective factor for OV. Furthermore, the fraction of infiltrating naive B cells, memory B cells, and other immune cells showed statistical differences between the high- and low- BNC1 expression groups through cell-type identification by estimating relative subsets of RNA transcripts (CIBERSORT) algorithm. Enrichment analysis showed that BNC1 may have a relationship with immune-related items in OV. By predicting the potential regulatory transcription factors (TFs) of BNC1 , friend leukemia virus integration 1 ( FLI1 ) may be a potential upstream TF of BNC1 . Corporately, a decreasing trend of BNC1 may serve as a tumor suppressor and prognostic biomarker in OV patients. Moreover, BNC1 may take part in immune-related pathways and influence the fraction of tumor-infiltrating immune cells.