Open AccessHsa-miR-30e-3p inhibits influenza B virus replication by targeting viral NA and NP genes
Author(s) -
Kritsada Khongnomnan,
Suthat Saengchoowong,
Oraphan Mayuramart,
Pattaraporn Nimsamer,
Trairak Pisitkun,
Yong Poovorawan,
Sunchai Payungporn
Publication year - 2020
Publication title -
experimental biology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 146
eISSN - 1535-3702
pISSN - 1535-3699
DOI - 10.1177/1535370220953151
Subject(s) - virus , virology , viral replication , biology , influenza a virus , gene , microrna , in silico , orthomyxoviridae , h5n1 genetic structure , genetics , medicine , covid-19 , infectious disease (medical specialty) , disease , pathology
Influenza B virus is a member of the Orthomyxoviridae family which can infect humans and causes influenza. Although it is not pandemic like influenza A virus, it nevertheless affects millions of people worldwide annually. MicroRNAs are small non-coding RNAs regulating gene expression at posttranscriptional level. They play various important roles in cellular processes including response to viral infection. MiRNA profiles from our previous study suggested that miR-30e-3p was one of the upregulated miRNAs that responded to influenza B virus infection. In this study, in silico prediction and in vitro investigation proved that this miRNA can directly target NA and NP genes of the influenza B virus and inhibit its replication. This finding might be useful for using miRNA as an alternative therapeutics for influenza virus infection.