Open AccessLabel-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy
Author(s) -
WeiChung Chen,
Yu-Jen Chen,
ShihTing Lin,
Wei-Han Hung,
Ming-Che Chan,
I-Chen Wu,
Ming-Tsang Wu,
ChieTong Kuo,
Subir Das,
Fu Jen Kao,
GuanYu Zhuo
Publication year - 2020
Publication title -
experimental biology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 146
eISSN - 1535-3702
pISSN - 1535-3699
DOI - 10.1177/1535370220934039
Subject(s) - second harmonic generation , submucosa , esophageal cancer , materials science , biomedical engineering , microscopy , cancer , pathology , biophysics , optics , medicine , laser , biology , physics
The issue of classifying esophageal cancer at various developmental stages is crucial for determining the optimized treatment protocol for the patients, as well as the prognosis. Precision improvement in staging esophageal cancer keeps seeking quantitative and analytical imaging methods that could augment histopathological techniques. In this work, we used nonlinear optical microscopy for ratiometric analysis on the intrinsic signal of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) from single collagen fibers only in submucosa of esophageal squamous cell carcinoma (ESCC). The blind tests of TPEF/SHG and forward (F)/backward (B) SHG were demonstrated to compare with the histology conclusion. The discussion of sensitivity and specificity was provided via statistical comparison between the four stages of esophageal cancer. To the best of our knowledge, this is the first study of using these two ratios in combination for staging ESCC.