Open AccessNeferine induces mitochondrial dysfunction to exert anti-proliferative and anti-invasive activities on retinoblastoma
Author(s) -
Jing Wang,
Yan Dong,
Qiuming Li
Publication year - 2020
Publication title -
experimental biology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.012
H-Index - 146
eISSN - 1535-3702
pISSN - 1535-3699
DOI - 10.1177/1535370220928933
Subject(s) - retinoblastoma , xenotransplantation , apoptosis , in vitro , downregulation and upregulation , malignancy , andrology , in vivo , immunohistochemistry , chemistry , medicine , microbiology and biotechnology , traditional medicine , pharmacology , biology , transplantation , biochemistry , gene
Retinoblastoma is common primary intraocular malignancy of infants and childhood. Neferine is a major bisbenzylisoquinoline alkaloid derived from the lotus plumule in Nelumbo nucifera . This study evaluated the mitigation role of Neferine on retinoblastoma in vitro and in vivo . Xenotransplantation model was established by injecting WERI-Rb-1 cells subcutaneously. Upon induction of retinoblastoma , mice were intraperitoneally injected with Neferine (0, 0.5, 1, 2 mg/kg) or ethanol every 3 days for 30 days. Tumor weight and tumor volume were measured every three days and compared between four groups. Then, mice were sacrificed and immunohistochemical examination was performed to compare Ki67, VEGF content between groups. WERI-Rb-1 cells were used for in vitro experiments and the anti-angiogenic role of Neferine was assessed by analyzing nodes/HPF number. In WERI-Rb-1 xenotransplantation model, compared with control group, 1 mg/kg Neferine treatment significantly inhibited tumor weight (0.39 ± 0.04 g vs. 0.25 ± 0.03 g, P < 0.05) and tumor volume (2163 ± 165 mm 3 vs. 1276 ± 108 mm 3 , P < 0.05) after 30 days. Compared with ethanol-injected mice, 2 μM Neferine treatment significantly enhanced apoptosis rate (2.1 ± 0.6% vs. 14.6 ± 2.6%, P < 0.05), accompany downregulation of Ki67 (0.09 ± 0.02% vs. 0.01 ± 0.004%, P < 0.05) and VEGF (0.28 ± 0.04% vs. 0.05 ± 0.03%, P < 0.05) expression. Additionally, 2 μM Neferine treatment significantly decreased JC-1 red/green percentage. High-dose Neferine could decrease retinoblastoma angiogenesis in association with a significant inhibition on tumor growth and invasion. These findings suggested that Neferine could be a new treatment or adjuvant against retinoblastoma.