Open AccessLactobacillus casei Extract Induces Apoptosis in Gastric Cancer by Inhibiting NF-κB and mTOR-Mediated Signaling
Author(s) -
Jeong Won Hwang,
Young Mi Baek,
Kyeong Eun Yang,
Hwa Seung Yoo,
Chong Kwan Cho,
Yeon Weol Lee,
Junsoo Park,
Chi Yong Eom,
Zee Won Lee,
Jong Soon Choi,
Ik Soon Jang
Publication year - 2012
Publication title -
integrative cancer therapies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 60
eISSN - 1552-695X
pISSN - 1534-7354
DOI - 10.1177/1534735412442380
Subject(s) - pi3k/akt/mtor pathway , apoptosis , lactobacillus casei , protein kinase b , cancer , cancer research , p70 s6 kinase 1 , cancer cell , signal transduction , nf κb , iκb kinase , phosphorylation , chemistry , biology , microbiology and biotechnology , medicine , biochemistry , fermentation
Lactobacillus casei extract (LBX) has been reported to prevent gastric cancer, but the underlying mechanism remains unclear. The proliferation and cell death of gastric cancer KATO3 cells were examined after treatment with LBX for various times and at various doses. LBX inhibited the growth of gastric cancer cells and induced apoptosis by inactivating NF-κB promoter activity. Apoptosis induced by LBX, however, is not directly associated with the intrinsic mitochondrial pathway. Immunoblot analysis revealed that LBX decreased the expressions of NF-κB and IκB. The reduced NF-κB levels led to the decreased phosphorylation of mTOR signaling components, such as PI3K, Akt, and p70 S6 kinase. These results showed for the first time that LBX induced apoptosis in gastric cancer cells by inhibiting NF-κB and mTOR-mediated signaling.