Open AccessA Comparison of Assay Performance Measures in Screening Assays: Signal Window, Z′ Factor, and Assay Variability Ratio
Author(s) -
Philip W. Iversen,
Brian J. Eastwood,
G. Sitta Sittampalam,
Karen L. Cox
Publication year - 2006
Publication title -
slas discovery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.907
H-Index - 75
eISSN - 2472-5560
pISSN - 2472-5552
DOI - 10.1177/1087057105285610
Subject(s) - window (computing) , measure (data warehouse) , statistics , signal (programming language) , computer science , computational biology , chromatography , biological system , mathematics , chemistry , data mining , biology , programming language , operating system
In this article, the authors compare the assay performance measures, signal window, Z' factor, and assay variability ratio. They examine their mathematical formulae for similarities and differences, describe their statistical sampling properties using the results of a computer simulation, and illustrate their use with example data. Based on these results, the authors recommend the Z' factor as a preferred measure of assay performance for screening assays and point out that none of these measures are adequate for characterizing concentration-response assays.